Rapid Bacterial Detection via an All-Electronic CMOS Biosensor

Rapid Bacterial Detection via an All-Electronic CMOS Biosensor

The timely and accurate diagnosis of infectious diseases is one of the greatest challenges currently facing modern medicine. The development of innovative techniques for the rapid and accurate identification of bacterial pathogens in point-of-care facilities using low-cost, portable instruments is essential. We have developed a novel all-electronic biosensor that is able to identify bacteria in...

SUPPLEMENTAL INFORMATION Increasing the detection speed of an all-electronic real-time biosensor

Increasing the detection speed of an all-electronic real-time biosensor.

Biosensor response time, which depends sensitively on the transport of biomolecules to the sensor surface, is a critical concern for future biosensor applications. We have fabricated carbon nanotube field-effect transistor biosensors and quantified protein binding rates onto these nanoelectronic sensors. Using this experimental platform we test the effectiveness of a protein repellent coating d...

Gold nanoparticle-labeled biosensor for rapid and sensitive detection of bacterial pathogens

BACKGROUND Escherichia coli O157:H7 is one of the major foodborne bacterial pathogens and also a biodefense agent. To ensure food safety and public health, it is very important to develop rapid methods for E. coli O157:H7 detection. In this study, we designed a nanoparticle-labeled biosensor for the rapid detection of E. coli O157:H7 in broth. RESULTS Magnetic nanoparticles (MNPs) were conjug...

Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens

Biological defense and security applications demand rapid, sensitive detection of bacterial pathogens. This work presents a novel qualitative electrochemical detection technique which is applied to two representative bacterial pathogens, Bacillus cereus (as a surrogate for B. anthracis) and Escherichia coli O157:H7, resulting in detection limits of 40 CFU/mL and 6 CFU/mL, respectively, from pur...